Fellow #zebrafish researchers, are you still using micro loader tips for microinjections? If so, where do you purchase them? Calibre Scientific sells them for $107 per box (96 tips). #Askzebrafish

Hi everyone! We were wondering if anyone in Europa would have some of these lines available and would be willing to share it with us?🐟🙏
Tg(ubi:cre)
Tg(isl2b:cre)
Tg(sox10:cre)
Or any similar cre lines that express in the CNS/retina?
#askzebrafish Zebrafish Rock!

Does anyone have a reporter line for investigating myelination in zebrafish and is willing to share it? #Askzebrafish #zebrafish

Any heat-shock protocols for Cre-induced recombination in adult #zebrafish #AskZebrafish

🤔 #AskZebrafish

#askzebrafish
In the market for a new fluorescent stereoscope to screen lines. Anyone have any scopes they like that work for dim reporters?

#AskZebrafish
Does anyone have either a mitfa:GFP plasmid or Tg(mitfa:GFP) zebrafish and are willing to share?

#AskZebrafish 🤔

#askZebrafish Zebrafish Rock!
A friend is trying to bulk sequence sorted cells. Qiagen RNeasy doesn't seem to get good yields, (a lot of material lost in the column. Does anyone have experience with RNA extraction kit for bulk seq of ZF sorted cells?

Hi
Zebrafish Rock!
,
EZS European Zebrafish Society
and other zebrafish lovers! I am looking for a ZED (Zebrafish enhancer detection) vector. Any ideas where to find it or who to ask?

Thank you so much in advance!

#zebrafish #AskZebrafish

Dear DevBio community, where shall I get 'v' shape #zebrafish injection molds #AskZebrafish . Any suggestions specifically in Europe 🙏🙏🙏 Zebrafish Rock!

#AskZebrafish Zebrafish Rock! for how long does photoconverted sox10:kaede stays on? What is the longest anyone has followed it?

Time for another weekend #PollForTheShoal !! 🤩 What is your favorite stage of #zebrafish ? It doesn't necessarily have to be the stage you currently work with 😎 #RockTheVote #AskZebrafish

#AskZebrafish Dear zebrafish users, does anyone try making Staygold (new version of GFP) transgenic lines? Do you think it can be better than mNeonGreen? If you have tried it or hear about something, could you share your experience? Thanks a lot for your help in advance!

#AskZebrafish Zebrafish Rock!

Does anyone have experience applying DL-based non-rigid 3D registration toolboxes (eg VoxelMorph, Mermaid) on zebrafish data? Looking for a trainable alternative to antsRegistration.

Query from Slack: Looking for a contact with any experience or knowledge with Minion Nanopore sequencing analysis of the #zebrafish gut microbiome 🙏 Any leads would be appreciated! #AskZebrafish

Query from Slack: Looking for liver specific ablation line such as Tg(fabp10a:CFP-NTR) or Tg(fabp10a:mcherry-NTR), preferably in the US but any lead would be appreciated 🤔 #AskZebrafish

Asking for some Twitter help (maybe Zebrafish Rock! can help) - Does anyone know at what specific stage of zebrafish oogenesis transcription stops? #AskZebrafish

Are there examples where knocking-out a transcription factor in differentiated cells reverts them back to progenitors or progenitor-like state? #developmentalbiology #AskZebrafish #StemCell

Can anyone recommend an antibody for aTub or a vital dye that successfully resolves microtubule cables in #zebrafish ?

We use Tg lines but trying to save the time of crossing to a mutant background if we can.

Thanks in advance 🙏🏼🐟

Zebrafish Rock! #AskZebrafish Amal Shawahny