We’re all wondering what the future of #spatialtranscriptomics (ST) has in store. Well, I think the near future will look a lot like the new 6000-plex dataset reviewed here (link to the original source at the end of this post).
SAMPLE
An entire 100 mm² intact 5 µm FFPE section
Comprehensive comparison of spatial tx platforms on FFPE samples presented by Human Wang involving 10x Genomics Xenium, NanoString CosMx and Vizgen MERSCOPE. #AGBT24
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✨さいぼNEWS！〖 速報 〗 🙌✨
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みなさま〜！！
この度、高解像度の新プラットフォームVisium HD初のデータ解析が終わりました👏㊗️🎉
ぜひ‼️
下記の画像をご覧ください🧑🏫✨
旧プラットフォームVisium FFPE v2との違いは一目瞭然です👀💫
気になる方はいますぐ🧬株式会社CyberomiX🧬｜ゲノミクスで未来を変えるカンパニー にご連絡を📢 ˎˊ˗
Patho-DBiT
Spatial #RNAWorld on FFPE section👹
Now tissues can be mapped by RNA variants or non-coding RNAs🤯
Whole transcriptome sequencing covers
mRNA
LincRNA
microRNA
sn/snoRNA
#AlternativeSplicing 105 events/85 genes/spot🤓
#RNAEditing
RNA #SingleNucleotideVariation
In this #OpenAccess study multiple IHC markers in 59 pleural #mesotheliomas were compared in paired specimens. Biopsies and FFPE pleural effusion cell blocks were concordant in most cases. #pulmpath #CytoHistoCorrelation #CytoJ
buff.ly/3PGb19X
STmut
Inferring+Visualizing #SomaticMutation #CopyNumberVariation Germline SNP/ #AllelicImbalance in Tumor #SpatialTranscriptomics
#SomaticMutation SNP
Frozen #Visium (sequencing-based ST needed) matched DNAseq needed
CNV: Also work with FFPE #Visium
matched DNAseq not required
Today's #TissueTuesday features a whole slide of a human FFPE #headandneck tumor labeled with 100+ antibodies. Imaged on the #PhenoCyclerFusion system, it reveals 14 distinct cell types and four unique tumor regions.
Inspired by @Aruthak's award-winning #cancerresearch , the
Today, we're featuring a figure that shows whole-slide #singlecell #spatialphenotyping of a human FFPE #headandneck squamous cell carcinoma with an ultrahigh-plex antibody panel using our #spatialbiology solution.
It reveals four distinct tumor regions with varying metabolic and
Super-resolved single-cell atlas of a human lymphoma FFPE tissue slide profiled using our latest Patho-DBiT technology and the novel pipeline iStar developed by Mingyao Li . Just too psyched to not share 🤩🤩
Wishing you a #ValentinesDay filled with striking red roses and heart-shaped tissues! 🌹
🌹The roses are made from FFPE breast tissue with a pattern of red and green stains.
💗The heart-shaped tissues are made from FFPE breast cancer using PhenoCycler-Fusion.
Conrad Lee
Small EVs as biomarkers for bladder cancer: Ken Pienta at JHU et al conducted RNA sequencing for FFPE tumor tissues and small EVs from matched tissue explants, urine, and plasma in BCa patients isevjournals.onlinelibrary.wiley.com/doi/full/10.10… #extracellularvesicles #exosomes #rna #liquidbiopsy #Vesiculab
Interested in automated sample prep for #singlenuclei from FFPE samples? Nathan Pereira, Product Manager at S2 Genomics will be presenting the new Singulator 200+ on stage at #FOGBoston , which automates the whole process. Register now: hubs.la/Q02y5zQq0
Very cool! Imaging mass cytometry on FFPE human kidney transplant samples Priya Alexander, MD American Journal of Transplantation
amjtransplant.org/article/S1600-…
For today's #TissueTuesday , we're sharing a human melanoma FFPE tissue stained w/ a 6-plex MOTiF PD-1/PD-L1 panel auto #melanoma kit & imaged on #PhenoImagerHT .
Learn about our kits crucial for melanoma samples in translational #immunooncology research. bit.ly/3wdqHuc
Today's #TissueTuesday showcases #prostatecancer FFPE tissue stained with an Opal 6-plex panel (CD4, CD8, CD19, CD21, DC-LAMP, and PNAd), revealing a Type V #tertiarylymphoidstructures (TLS).
Thank you, Jessica Duarte, for using our #PhenoImager solution to generate this
