TET1-mediated germline reprogramming & sperm methylome patterning ft. Rexxi Prasasya, Blake Caldwell, Zhengfeng Liu, Songze Wu, Dr. Marisa Bartolomei (@PennCDB), Johanna Fowler, Steven Cincotta, Diana Laird, Kohli Lab (@PennMedicine) & N. Adrian Leu (@PennVet) tinyurl.com/49rferp4

Congrats to Rexxi Prasasya on work showing importance of TET1 to germline reprogramming beyond imprinted & meiosis genes--sperm HMRs. Thanks to collabs-Kohli Lab & Diana Laird Iterative oxidation by TET1 is required for reprogramming of imprintin..sciencedirect.com/science/articl…

Base editors are transformative precision genome editing tools. But do we really understand how Cas9 & DNA deaminases work together to shape editing outcomes? See how Kiara tackled this Q below to introduce both a powerful diversifying editor & new mechanistic model for BEs! 🧵👇

It was a joy to be part of this collaborative effort and expand our own interest in base editors to the world of RNA BEs. Congrats to Hugo Medina-Muñoz and all the other authors!

Read about using rBEs in PRINTER to study RNA-protein interactions 👇
nature.com/articles/s4146…

Registration is now open for the 5th International Conference On Base Editing, Prime Editing & Related Enzymes (aka Deaminet 2024), held January 17- 19, 2024, in San Diego, California at the Estancia La Jolla Hotel.
Precision gene editing + San Diego =✌️
cancerresearch.uci.edu/seminars-and-e…

Congrats to Michael Cory, PhD for being awarded the Kanefield Prize for Research Penn BGS! Michael’s dissertation work on bacterial DNA repair via the SOS response pathway provides key insights to help combat antibiotic resistance, a major concern among #CysticFibrosis patients.

We had a wonderful time at this year’s Penn Epigenetics retreat! So very many great talks and posters 🤩 Shoutout to Christian Loo on winning a poster prize!

Hey BBCB Graduate Group UPenn Dept of Biochem & Biophys labs, did you hear? We’re COOL 😎. Be like us! #GetVaccinated !
And then #RideTheSHOTWAVE by posting a picture of your bandaid to help others get access to vaccines!

Loving this EpiGenie feature on our new DM-Seq method for direct enzymatic sequencing of 5-methylcytosine...'a DM you'll want to slide into.' We agree! Slide on over to the link below 🛝👇. And then go #RunDMSeq 🧬!

epigenie.com/dm-seq-a-dm-yo…

Congrats to Penn Arts & Sciences rising senior & Kohli lab undergraduate researcher Jasleen Gill for being chosen as a Roy and Diana Vagelos Science Challenge Award recipient! This award is a testament to her outstanding work in the sciences. We’re super proud of you, Jasleen Gill! 🏆🎉👩‍🔬

Congratulations to our newest PhD candidate Aleksia Barka for successfully passing her BBCB Graduate Group prelim exam (and doing it before officially starting grad school, no less)!

Being able to limit the size & control the copy number of genomic information in a human artificial chromosome would be a major step forward...and here it is! 🧑‍🦰🤖🧬 See below for must-read work from the lab of Ben Black UPenn Dept of Biochem & Biophys Penn Center for Genome Integrity 👇 biorxiv.org/content/10.110…

Excited to share our new paper ACS Publications! We melded our biochemistry expertise + CisnerosResearch computational expertise to turn a natural DNA methyltransferase (MTase) into a neomorphic DNA carboxy-MTase with novel substrate selectivity🧬. See the tweetorial below for more👇

Congratulations to Wumesh KC (Penn Cardiology), Ted Kreider (@WeissmanLab) & Elizabeth Traxler (@PennCancer) on being selected as members of the 1st cohort of the @Penn Measey Scholars in Molecular Medicine Program!🎉 Learn more➡️ molmedscholars.org

Novel DM-Seq Method Profiles 5mC at Single-Base Resolution Using Nanograms of DNA

A new, precise, and efficient DNA sequencing method, direct methylation sequencing (DM-Seq), may lead to easier testing and earlier cancer detection...Read more: ow.ly/7PlA50OQn25

For an at-a-glance rundown of some of the key highlights of our DM-Seq paper, check out the Tweetorial by Tong Wang, MD, PhD linked below! #runDMSeq

Many thanks to our team of collaborators, particularly Integrated DNA Technologies & Hao Wu | 吴昊, who helped DM-Seq grow into a novel base-resolution sequencing method with potential for clinical impact. Thanks also to National Human Genome Research Institute for support & Genome Tech Dev Coordinating Center for featuring DM-Seq in the video below.

Can the enzymes that write #DNA modifications be turned into readers of #epigenetic modification states? Excited to share the answer is 'yes!' Read more about our all enzymatic & nondestructive method for mapping DNA methylation in Nature Chemical Biology #runDMSeq nature.com/articles/s4158…