How does the #kidney filter 1500 liters of blood every day without loosing proteins?
Our #cryoET data of kidney tissue by #waffleMethod show that the slit diaphragm, which links #podocytes in the #glomeruli , looks like a fishnet!
biorxiv.org/content/10.110…

STOPGAP, an open-source package for subtomogram averaging, is presented together with detailed descriptions of the image-processing algorithms that it uses @vanderbiltu Structural Biology IUCr #STOPGAP #CryoET #OpenSource doi.org/10.1107/S20597…

Using the #VideoGame engine #UnrealEngine 5 to render #protein #structures from #CryoET inside #cells at the #atomic level:

Preprint: doi.org/10.1101/2023.1…
Tutorial: blake.bcm.edu/emanwiki/EMAN2…

#SciArt #CompChem #bioinformatics #EMAN2 #UE5 #DIYbio #education #MINT #STEM #SciComm

Bacteria can be pirates too... 🏴‍☠️ Let us introduce you to Captain Aureispira ⛵️ A bacterial pirate ship loaded with canons 💉 and grappling hooks 🪝🌴predating on other bacteria 🦠 Check the preprint: biorxiv.org/content/10.110… #predation #T6SS #T9SS #cryoEM #cryoET #teamtomo 🧵1/6

We are excited to announce the publication of high confidence 3D template matching for #cryoET
Hummer Lab Beck Laboratory Jan Philipp Kreysing Katharina Geißler Stefan Schäfer Patrick Hoffmann Max Planck Institute of Biophysics CZI Science

Paper's link: nature.com/articles/s4146…

See it in action in our short video!

Had a great day at the Electron Microscopy Facility Université de Lausanne learning FIB-milling on the Aquilos 2!

Hopefully preparing some nice lamella for cryoET. #teamtomo

👋 in the new and notable section to see Ed Twomey’s talk - to conform with the no photo rule please see a pic of a blue screen 😂 #BPS2024

If you're interested in nuclear pore complexes and how we used in situ cryoET to figure out how cells ensure their correct orientation, come visit me at my poster P2038 (B315) at 12:15 or come to my talk in the CryoEM and CryoET subgroup at 5:15 (Room 253B)! #cellbio2023

🤯🤯🤯 Discovering the true form of the kidney filter (slit diaphragm) using cryoET ! Contrary to common belief, it resembles a complex mesh rather than simple finger-like structures. The molecular architecture of the kidney slit diaphragm 🤯🤯biorxiv.org/content/10.110…

High-confidence 3D template matching for #cryoET published by Sergio Cruz-León & coworkers from our Hummer Lab & Beck Laboratory in Nature Communications!
Check out the fast, automated, objective and comprehensive feature identification towards visual proteomics👉link.springer.com/article/10.103…

We updated our comparative study of G0 & proliferating fission yeast nuclei. The in situ cryo-ET is redone with cryolamellae + K3-GIF + 3-D classification. Canonical nucleosomes are rare in all cell types! Congrats to Zhi Yang, Shujun Cai, et al. biorxiv.org/content/10.110…

The #CryoET structure of toroid assembly formation of FtsZ filaments driven by ZapD as the cross-linker. elifesciences.org/reviewed-prepr…

The first keynote of #BCI2024 , Vicki Gold from University of Exeter presents the fascinating journey of Vicki Gold ‘s work on bacterial motility and structural insights into type IV pili 🦠🧫 #cryoEM #cryoET #womeninSTEM

A little over 2 months left until delivery of the Microscopy & Spectroscopy #PFIB for #cryoET sample prep to @mpibp. Since last week, the room has a floor. And field compensation cable ducts. Still Deutschland-Tempo but 🐌 > 🐢.

In our new #cryoFIB #cryoET preprint we imaged actin filaments in very fast moving Plasmodium falciparum sporozoites. We shed some light on the mechanism of gliding motility and find unexpectedly long and large actin bundles in the cytoplasm and nucleus!

biorxiv.org/cgi/content/sh…

Inspired by a childhood filled with electronics + a fascination with shiny instruments, UC Berkeley’s Holger Müller is #ImagingTheFuture by building a laser phase plate to improve #CryoET images

🚨 New paper alert 🚨 I am so happy to share the first paper of my postdoc in Mizuno Lab . Here we hope to summarize the cellular landscape of neurons thru the lens of #cryoET . Hope you enjoy. authors.elsevier.com/c/1ivnv3PA3sYq…

Excited to be hosting #cryoET #TeamTomo superstar Dr. Elizabeth Villa for the Beadle & Tatum named lecture today!! Can’t wait to hear about all of the exciting new work coming out of Elizabeth Villa 🤩❄️🔬

We are excited to share our preprint, a #teamtomo voyage into human brain with 'In situ cryoET of β-amyloid and tau in post-mortem Alzheimer's disease brain'. biorxiv.org/content/10.110…