🔬Target spotlight: Polyphosphoinositide phosphatase, encoded by #FIG4 gene. Identify a high-performing FIG4 antibody by exploring this antibody characterization report, available on our Zenodo community➡️
zenodo.org/records/108149…

10 leading antibody manufacturers, YCharOS and Structural Genomics Consortium (SGC) team up to agree on a (reagent) antibody characterization platform/protocol. If more folks adopt this, we’d be well on our way to more reproducible biomedical research!

protocolexchange.researchsquare.com/article/pex-26…

🔬This week we're featuring two #hnRNP targets: hnRNP A1 and hnRNP A2/B1. We've characterized commercial #antibodies , using our scalable and #reproducible protocol. Find the results on our Zenodo community➡️
hnRNP A1:doi.org/10.5281/zenodo…
hnRNP A2/B1:doi.org/10.5281/zenodo…

Thanks for sharing Institute for Protein Innovation !
Great and quick read by aled edwards and Mona AlQazzaz on the blinding issue of #antibody use in research. They go over how we first identified the issue, the advantages of an #openscience community in addressing it and plans moving forward⏩

Our public good effort to characterize commercial recombinant antibodies using knock-out cells as controls identified excellent antibodies for PPP2R5D, a protein linked to autism. Good reagents yield reproducible research!

🔬This week's protein target spotlight: PLC-gamma-2. Eleven commercial antibodies were evaluated by WB, IP and IF. The results are now published on both our #openaccess platforms:
F1000 gateway: doi.org/10.12688/f1000…
Zenodo community: doi.org/10.5281/zenodo…
#openscience #PLCG2

Our thanks to The Transmitter for featuring an article by our own aled edwards and Mona AlQazzaz!

We found a major flaw in the antibody reagents used in thousands of neuroscience experiments — and we’re trying to fix it. #OpenScience #neuroscience

thetransmitter.org/open-neuroscie…

Quantifying the performance of 614 #antibodies for 65 human proteins demonstrates the scale of the antibody liability issue.
Good news? we CAN help identify a successful antibody for your protein of interest.
➡️doi.org/10.7554/eLife.…
#antibodyvalidation #reproducibility

In case you missed it, the recording is now available ➡️responsibleresearchinpractice.co.uk/2023/12/13/gui…

There is still time to register for our December #FREE #webinar on Wed 13th Dec.
Join Dr Harvinder Virk and Dr Nikki Osborne for this FREE 'Guide to choosing and using #antibodies to improve the #reproducibility of research' webinar.

buff.ly/48vps84

Thanks again to YCharOS for organizing this session

A parting thought on choosing antibodies: know the antibody’s provenance; Who made it? When? Did they use it themselves? Who did the characterization? Was it made ‘artisan-style’ or in a big project? These things matter.

Big thank you to everyone who joined us on Tuesday at #CellBio23 ! It was inspiring to witness the collective effort to address the #antibodyliability crisis. Looking forward to the continued momentum and progress of this collaborative initiative.

Harvinder Virk Emory School of Medicine abcam GeneTex Cell Signaling Technology Douglas W. Houston University of Iowa DSHB Andrea Marat JCellBiol Awareness is a good start. Now it’s “all oars in the water,” host Kahn says 🚣🚣
Thanks for a great session YCharOS & American Society for Cell Biology! Want to know more? Start with our recent blog on cleaning up the Ab landscape & stay in touch! #CellBio2023 proteininnovation.org/2023/09/ipi-su…

Find us at the American Society for Cell Biology #CellBio2023 session on #antibodyvalidation tomorrow, Dec. 5 from 10:30 to noon! IPI’s Rob Meijers will be sitting down with friends from YCharOS, OnlyGoodAntibodies, DSHB & industry, too. Come watch!
plan.core-apps.com/ascbembo2023/e…