Arno Alpi is telling us about Non-canonical substrate recognition by E3 ligases. See one of their recent papers: febs.onlinelibrary.wiley.com/doi/10.1002/18… #ubfriends2024

Was really great catching up with Ruben Fernandez and Cole Sitron from Aligning Science Across Parkinson’s Team Harper Wade Harper at the #ubfriends2024 symposium in wonderful Vienna. Max Planck Institute of Biochemistry (MPIB) Uni Göttingen Harvard Med Cell Bio

Very happy to finally finish this paper and the corresponding code and tutorial. Grateful to all those who helped make this happen. Hopefully it will contribute to making #cryoET more accessible. #teamtomo

biorxiv.org/content/10.110…

Beyond excited to share our story on CUL9- a giant cullin-RING AND RBR E3 ligase. We uncovered its hexameric structure via #CryoEM and got surprised by NEDD8 along the way!
Amazing collab with LinusHopf Max Planck Institute of Biochemistry (MPIB)

Check out the full story NatureStructMolBiol nature.com/articles/s4159…

Wow! CUL9 is a GIANT E3 ubiquitin ligase!  See unique cullin-RING-RBR all-in-one. +/- NEDD8.

Now online in Nature Structural & Molecular Biology by Schulman Department

❕nature.com/articles/s4159…

#ubiquitin #E3ligase #cryoEM
Daniel Horn-Ghetko LinusHopf Matthias Mann Lab NatureStructMolBiol

Two celebrations in a row: After defending my PhD on Monday, the 2nd paper is online today. Thanks to all the fantastic people who supported my journey and made it so much fun.
Check out our new paper on the cullin-RBR-E3 fusion protein CUL9: nature.com/articles/s4159…
#PhD #cryoEM

doi.org/10.1038/s41594…
How to build a proteasome from scratch. A little serendipity and a lot of work from Frank Adolf and Jiale Du in the Schulman lab with their HMS collaborators.

See how #proteasomes assemble, that #chaperones progressively fold into an internal scaffold with successive subunit additions, and how the active site-adjacent regions are restructured through fusion of two halves.
❕nature.com/articles/s4159…
#cryoem Aligning Science Across Parkinson’s Harvard Med Cell Bio

Molecular machines rule! Frank Adolf & Jiale Du in Brenda Schulman lab Max Planck Institute of Biochemistry (MPIB) , John Hanna (BWH/Harvard), & Ellen Goodall Wade Harper Harvard Med Cell Bio visualize chaperone-mediated stepwise assembly of the 20S proteasome by cryo-EM Aligning Science Across Parkinson’s nature.com/articles/s4159…

You are a highly talented researcher?
The departments of Brenda Schulman and Matthias Mann are looking for you!

Check out the #job offer:
❕apply as soon as possible
❕mpg.de/21767203/phd-s…

#hiring #JobAlert Matthias Mann Lab
#ubiquitin #E3ligase #UPS #massspec #structural_biology

Back after an Easter X-break with a new paper from PhD student Annette Gross, Arno Alpi and the Schulman team explaining how the huge spectrum of WDR26 mutations is involved in #Skraban -Deardorff intellectual disability syndrome

Please RT: We have an open PhD position in my lab at Max Perutz Labs Vienna. We are interested in the mechanistic understanding of gene expression regulation via small RNAs and the nuclear RNA quality control pathways. Focus: biochemistry and structural biology. Questions? Write me!

Mechanistically insightful work from the labs of Gary Kleiger and Brenda Schulman unveils a “synergy loop” in UBE2Rs, connecting the CRL, donour, and acceptor ubiquitins, endowing such complexes with near perfect catalytic efficiency. nature.com/articles/s4159…

bit.ly/3I4ZpZd The biochemical complexity of the (range of) ubiquitin transfer reactions is just insane. Here is a new and generalizable approach, sure to useful to the PROTAC industry. From the Schulman group and Gary “The Governor” Kleiger Munich-Las Vegas axis

Now out in Molecular Cell! High-res structure showing direct #ubiquitylation of a #TPD substrate!
❕ cell.com/molecular-cell…

#cryoEM UNLV College of Sciences Joanna Liwocha @DanielS2012197

Complementary study from Alessio Ciulli & Charlotte Crowe biorxiv.org/content/10.110…
Centre for Targeted Protein Degradation UoD Life Sciences

Now out in Molecular Cell! High-resolution structure showing direct ubiquitylation of a #TPD substrate! And much more, check out 👉shorturl.at/jrsBG #cryoEM Max Planck Institute of Biochemistry (MPIB) UNLV College of Sciences St. Jude Research Daniel Scott rajan prabu

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See cullin-RING E3s forge #ubiquitin chains on substrates and degrader-recruited neo-substrate at high-res! Millisecond polyubiquitylation! #cryoEM #TPD NatureStructMolBiol
❕nature.com/articles/s4159…

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See how CRLs forge ubiquitin chains on substrates - and degrader-recruited neo-substrate - at high-res! Millisecond polyubiquitylation! #cryoEM #TPD NatureStructMolBiol Max Planck Institute of Biochemistry (MPIB) UNLV College of Sciences
👉shorturl.at/eFS68
Related exciting work from Alessio Ciulli shorturl.at/hNSU5

Tomorrow we're hosting our annual Axel Ullrich Lecture with Jonathan Weissman's Lab ! Make sure to join us at 1pm in our lecture hall in Martinsried!

❕further information about the lecture: biochem.mpg.de/aul

See you there!